The decision, which comes into effect on November 29, will affect passengers who have travelled through South Africa, Namibia, Lesotho, Eswatini, Zimbabwe, Botswana or Mozambique over the past 14 days.
The General Civil Aviation Authority and the National Emergency Crisis and Disasters Emergency Management Authority made the announcement on Friday.
Flights will continue to transport passengers from the UAE to these seven countries for now.
Citizens of the UAE, members of diplomatic missions, official delegations and those with golden residence visas are excluded from the new rules.
Those who are exempt will need to present a negative Covid-19 test obtained within 48 hours of departure, take a rapid PCR test at the airport within six hours of departure and perform another PCR test at the airport when arriving in the UAE.
Official delegations will be required to quarantine at the airport until their test results are received and then will be able to continue their mission in the UAE without the 10-day quarantine.
UAE citizens are prohibited from travelling to the affected countries unless they are involved in emergency treatment cases, are members of official delegations or are on scholarships.
The announcement comes after the WHO designated the new B. 1.1.529 Omicron strain as a “variant of concern”.